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Choline kinase. Choline kinase catalyzes the first committed step in the phosphorylation of choline to form PC. This reaction is reversible, producing PCho from PC. It is the most specific of all choline metabolic enzymes, and has only one unique substrate, PC. In a so-called “forward” reaction, it specifically phosphorylates choline; in a “reverse” reaction, it dephosphorylates phosphocholine and produces choline. The enzyme is also called phosphocholine-specific phosphokinase.
In the phosphorylation reaction, choline kinase uses choline as an energy-rich phosphate donor. The activity of choline kinase is approximately 10-fold higher in the brain than in the liver.
Choline is transported into the cell by a sodium-dependent transporter. (Aspartate and glutamate transporters are also considered to have choline-transport activity). In neurons the transporter is called choline transporter type 1 (CHT1) and in liver and kidney cells it is called choline transporter type 5 (CHT5). A second choline transporter, CHT8, is only found in the retina.
In brain cells, choline is thought to be involved in brain function by maintaining an appropriate level of the neurotransmitters acetylcholine (ACh) and (S)-adenosyl-L-methionine (SAM). SAM is made in the cell from choline and (S)-methionine. ACh is made from SAM and acetyl coenzyme A (CoA) by the enzyme choline acetyltransferase.
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